primary monoclonal antibodies anti-p21  (Cell Signaling Technology Inc)

Journal: BMC Cancer

Article Title: Novel chemotherapeutic agent FX-9 activates NF-κB signaling and induces G1 phase arrest by activating CDKN1A in a human prostate cancer cell line

doi: 10.1186/s12885-021-08836-y

Figure Lengend Snippet: Reactome top 15 enriched signaling pathways after 6-h exposure of FX-9

Article Snippet: This was followed by incubation with mouse monoclonal primary antibodies p21 Waf1/Cip1 (diluted 1:100, sc-6246, Santa Cruz Biotechnology, Inc., Dallas, TX, USA,), E2F-1 (diluted 1:200, sc-251, Santa Cruz Biotechnology, Inc.,), PAI-1 (diluted 1:50, sc-5297, Santa Cruz Biotechnologys, Inc.), and rabbit monoclonal primary antibody NFkB2/NFkB p100 (diluted 1:200, JM82–03, Novus Biologicals, LLC, Littleton, CO, USA) at 4 °C over night.

Techniques: Protein-Protein interactions, Binding Assay, Activation Assay

Journal: BMC Cancer

Article Title: Novel chemotherapeutic agent FX-9 activates NF-κB signaling and induces G1 phase arrest by activating CDKN1A in a human prostate cancer cell line

doi: 10.1186/s12885-021-08836-y

Figure Lengend Snippet: ICC-staining comparing control cells with cells after 12-h or 24-h exposure to 5 μM FX-9

Article Snippet: This was followed by incubation with mouse monoclonal primary antibodies p21 Waf1/Cip1 (diluted 1:100, sc-6246, Santa Cruz Biotechnology, Inc., Dallas, TX, USA,), E2F-1 (diluted 1:200, sc-251, Santa Cruz Biotechnology, Inc.,), PAI-1 (diluted 1:50, sc-5297, Santa Cruz Biotechnologys, Inc.), and rabbit monoclonal primary antibody NFkB2/NFkB p100 (diluted 1:200, JM82–03, Novus Biologicals, LLC, Littleton, CO, USA) at 4 °C over night.

Techniques: Control, Staining

Journal: Oncology reports

Article Title: MicroRNA-134-3p inhibits ovarian cancer progression by targeting flap structure-specific endonuclease 1 in vitro.

doi: 10.3892/or.2020.7844

Figure Lengend Snippet: Figure 2. miR-134-3p facilitates cell apoptosis and induces cell cycle arrest at the G0/G1 phase in SKOV-3 and OVCAR-3 cells. SKOV-3 and OVCAR-3 cells were transfected with miR-134-3p mimic or NC mimic. (A) TUNEL assay was performed to assess cell apoptosis (scale bar, 200 µm). (B) Western blot analysis was used to assess the protein expression levels of Blc-2, Bax, cleaved caspase-3 and cleaved caspase-9. (C) Flow cytometry assay was performed to assess the cell cycle. (D) Western blot analysis was used to assess the protein expression levels of cyclin D1, CDK2 and p21. The data are presented as the mean ± SD (n=3). *P<0.05 and **P<0.01 vs. NC mimic. PCNA, proliferating cell nuclear antigen; miR, microRNA; NC, negative control.

Article Snippet: The membranes were then blocked with TBS-Tween (TBST; 0.1% Tween-20) containing 1% skim milk powder at room temperature for 1 h, and then incubated with rabbit monoclonal primary antibodies against human p21 (1:1,000; cat. no. 2947S), cyclooxygenase-2 (Cox-2; 1:1,000; cat. no. 12282T), matrix metalloproteinase (MMP)2 (1:1,000; cat. no. 40994S), MMP9 (1:1,000; cat. no. 13667S), cyclin d1 (1:1,000; cat. no. 55506S), CdK2 (1:1,000; cat. no. 2546S), Bax (1:1,000; cat. no. 5023S), cleaved caspase-3 (1:1,000; cat. no. 9654S), cleaved caspase-9 (1:1,000; cat. no. 20750S), Bcl-2 (1:1,000; cat. no. 4223S), β-actin (1:1,000; cat. no. 4970T) and FEN1 (1:2,000; cat. no. 82354S) (all from Cell Signaling Technology, Inc.) at 4 ̊C overnight.

Techniques: Transfection, TUNEL Assay, Western Blot, Expressing, Flow Cytometry, Negative Control